Identification, Morphological Characterization and Distribution of Fusarium species in Moroccan Wheat

Main Article Content

Houda Khalifi
Fatiha Bentata
Mustapha Labhilili
Soukaina Hammoumi
Sanae Karim
Mouaaid Abdelali
Najiba Brhadda
Rabea Ziri

Abstract

Wheat Fusarium root rot is a common worldwide disease that occurs in a wide range of environmental and temperature conditions. Understanding morphological and molecular variations, as well as the geographic distribution of the fungi responsible is very important for a better disease control. Seventy-two Fusarium isolates were recovered from infected wheat plants collected in seven wheat production regions of Morocco. Five different species of Fusarium were isolated: F. avenaceum, F. culmorum, F. sambucinum, F. equiseti, and F. graminearum. For laboratory cultures, the potato dextrose agar is commonly used and gives good results for growth rate and morphological typical structures of the pathogen. In this study, Malt-extract and Potato dextrose Agar media were tested. The latter had shown higher growth rate for most of the Fusarium species and diverse morphological structures and pigmentations. The most common species in the regions of study is F. culmorum (59.72%), present in all of the studied areas followed by F. equiseti (11.11 %) and F. graminearum (8.33%) which were mainly restricted to North-Western Morocco, while F. sambicunum and F. avenaceum, with a percentage of 1.39% each, were only found in Central-Morocco. 

Downloads

Download data is not yet available.

Article Details

Section

Articles

Author Biographies

Houda Khalifi, Laboratory of Plant and Animal Production and Agro-industry productions, Faculty of Science, Ibn Tofail University, Av l’université, Kenitra, Morocco. 

National Institute of Agricultural Research (INRA), Regional Research Center for Agricultural of Rabat, Av Mohamed Belarbi Alaoui, B.P. 6356, Institutes, Rabat, Morocco 

Soukaina Hammoumi, Laboratory of Plant and Animal Production and Agro-industry productions, Faculty of Science, Ibn Tofail University, Av l’université, Kenitra, Morocco. 

National Institute of Agricultural Research (INRA), Regional Research Center for Agricultural of Rabat, Av Mohamed Belarbi Alaoui, B.P. 6356, Institutes, Rabat, Morocco

Sanae Karim, National Institute of Agricultural Research (INRA), Regional Research Center for Agricultural of Rabat, Av Mohamed Belarbi Alaoui, B.P. 6356, Institutes, Rabat, Morocco 

Laboratory of Spectroscopy, Molecular Modeling, Materials, Nanomaterials, Water and Environment, Faculty of Sciences, Mohammed V University of Rabat, Av Ibn Battouta, BP1014, Agdal, Morocco.

How to Cite

Khalifi, H., Bentata, F., Labhilili, M., Hammoumi, S., Karim, S., Abdelali, M., Brhadda, N., & Ziri, R. (2025). Identification, Morphological Characterization and Distribution of Fusarium species in Moroccan Wheat . Tropical Journal of Natural Product Research (TJNPR), 9(5), 2307 – 2313. https://doi.org/10.26538/tjnpr/v9i5.59

References

1. Sharma S, Schulthess AW, Bassi FM, Badaeva ED, Neumann K, Graner A, Özkan H, Werner P, Knüpffer H, Kilian B. Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm. Biology (Basel). 2021;10(10):982.

2. Briak H, Kebede F. Wheat (Triticum aestivum) adaptability evaluation in a semi-arid region of Central Morocco using APSIM model. Sci Rep. 2021;11(1):23173.

3. Chibi A, Hassaine A. Essential Oil of Eucalyptus polybractea (L.): Chemical Composition, antifungal, insect repellent and insecticidal activities. Trop J Nat Prod Res. 2023;7(10):4160–5.

4. Noble R, Coventry E. Suppression of soil-borne plant diseases with composts: A review. Biocontrol Sci and Technol. 2005;15(1):3–20.

5. Semple ERL, Frio AS, Hicks PA, Lozare JV. Mycotoxin prevention and control in foodgrains. [Online]. 2011; [cited 2024 Dec 10]. Available from: https://www.fao.org/4/x5036e/x5036E00.htm

6. Abu El Samen FM, Alsawalha I, Alananbeh KM, Al-Karablieh N, Al-Abdallat AM. Evaluation of endophytic bacteria from durum wheat on Fusarium root and crown rot disease (Fusarium culmorum) under drought stress. Agronomy. 2024;14(12):2912.

7. Mitter V, Zhang MC, Liu CJ, Ghosh R, Ghosh M, Chakraborty S. A high-throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm. Plant Pathol. 2006;55(3):433–41.

8. Goswami RS, Kistler HC. Heading for disaster: Fusarium graminearum on cereal crops. Mol Plant Pathol. 2004;5(6):515–25.

9. Petronaitis T, Simpfendorfer S, Hüberli D. Importance of Fusarium spp. in wheat to food security: A global perspective. In: Scott P, Strange R, Korsten L, Gullino ML, editors. Plant Diseases and Food Security in the 21st Century [Internet]. Cham: Springer International Publishing; 2021 [cited 2025 Mar 4]. p. 127–59. Available from: https://doi.org/10.1007/978-3-030-57899-2_7

10. Lyamani A. Wheat root rot in west central Morocco and effects of Fusarium culmorum and Helminthosporium sativum seed and soil-borne inoculum on root rot development, plant emergence and crop yield [Internet]. 1988 [cited 2023 May 26]. Available from: https://www.proquest.com/openview/beaf0ab7dcfbee2202b40b744f4d62ab/1?pq-origsite=gscholar&cbl=18750&diss=y

11. Oslane I, Brahim EY, Ouabbou H, Younssi E, R. T. Assessment of the severity and tolerance of a Moroccan collection of durum wheat to root rot disease. (Évaluation de la sévérité et de la tolérance d’une collection marocaine du blé dur aux pourritures racinaires) Rev Maroc Prot Plantes. 2014; N 5:17–30.

12. Khalifi H, Bentata F, Bouarda J, El Aissami A, Niya I, Kahama Issa A, Maafa I, Hammoumi S, Karim S, Ibriz M, Amrani Joutei K, Brhadda N, Ziri R, Labhilili M. Molecular characterization of new causative agents of root rot of wheat in Morocco. Agron Res.2024;22(2), 832–844.

13. Soudi G. Wheat Fusariosis in Morocco: Characterization, Mapping, and New Control Methods (Fusariose du blé au Maroc: Caractérisation, cartographie et nouvelles méthodes de lutte). [thesis].2019.

14. Manshor N, Azuddin NF, Mohd MH, Nor NMIM, Zakaria L. Characterization and mycotoxin analysis of Fusarium spp. from highland areas in Malaysia. Malays Appl Biol. 2022;51(3):89–106.

15. Choi YW, Hyde K, Ho W, Choi Y, Ho A. Single spore isolation of fungi. Fungal Divers. 1999; 3:29-38.

16. Trabelsi R, Sellami H, Gharbi Y, Krid S, Cheffi M, Kammoun S, Dammak M, Mseddi A, Gdoura R, Triki MA. Morphological and molecular characterization of Fusarium spp. associated with olive trees dieback in Tunisia. 3 Biotech. 2017;7(1):28.

17. Younesi H, Darvishnia M, Bazgir E, Chehri K. Morphological, molecular and pathogenic characterization of Fusarium spp. associated with chickpea wilt in western Iran. J Plant Prot Res. 2021;61(4):402–413.

18. Leslie JF, Summerell B, Bullock S. The Fusarium Laboratory Manual | Wiley-Blackwell; 2006. 416 p. ISBN 978-0-813-81919-8. [cited 2023 May 26]. Available from: https://www.wiley.com/en-gb/The+Fusarium+Laboratory+Manual-p-9780813819198

19. Winter M, Samuels PL, Otto-Hanson LK, Dill-Macky R, Kinkel LL. Biological control of Fusarium crown and root rot of wheat by Streptomyces isolates – It’s Complicated. Phytobiomes J. 2019;3(1):1–81.

20. Miedaner T, Juroszek P. Climate change will influence disease resistance breeding in wheat in Northwestern Europe. Theor Appl Genet. 2021;134(6):1771–85.

21. Błaszczyk L, Ćwiek-Kupczyńska H, Hoppe Gromadzka K, Basińska-Barczak A, Stępień Ł, Kaczmarek J, Lenc L. Containment of Fusarium culmorum and its mycotoxins in various biological systems by antagonistic Trichoderma and Clonostachys strains. J Fungi (Basel). 2023;9(3):289.

22. El Yacoubi H, Hassikou R, Alain B, Atmane R, Douira A. Root rot fungal complex of soft wheat in northwestern Morocco. (Complexe fongique de la pourriture racinaire du blé tendre au nord-ouest du Maroc). Bull Soc Pharm Bordeaux. 2012; 151:35–48.

23. Demeke T, Clear RM, Patrick SK, Gaba D. Species-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis. Int J of Food Microbiol. 2005;103(3):271–84.

24. Özer G, Erper İ, Yıldız Ş, Bozoğlu T, Zholdoshbekova S, Alkan M, Tekin, F., Uulu, T. E., İmren, M., Dababat, A. A., & Derviş, S. Fungal pathogens associated with crown and root rot in wheat-growing areas of Northern Kyrgyzstan. J Fungi (Basel). 2023;9(1):124.

25. DonnéesMondiales. Climat: Rabat-Salé-Zemmour-Zaër in Morocco [Online]. [Cited 2023 May 25]. Available from: https://www.donneesmondiales.com/afrique/maroc/climat-rabat-sale-zemmour-zaer.php

26. Shukla A, Sharma D, Sharma M, Tarafdar A, Gupta M. First report of Fusarium equiseti causing crown and root rot of cucumber in India. J Plant Pathol. 2022;104(2):875–875.

27. Jia R, Yang J, Hao J, Wang S, Wu J, Lin K, Chen Z, Zhang Y. First report of root rot caused by Fusarium equiseti on Syrian rue (Peganum harmala) in China. Plant Dis. 2024; 109(1):234.

28. Ezrari S, Lahlali R, Radouane N, Abdessalem T, Lazraq A. First report of Fusarium equiseti causing pre- and postharvest fruit rot on zucchini in Morocco. J Plant Pathol. 2019;102(1), 251-251.

29. Ezrari S, Lahlali R, Radouane N, Tahiri A, Asfers A, Boughalleb N, Amiri S, Lazraq A. Characterization of Fusarium species causing dry root rot disease of citrus trees in Morocco. J Plant Dis Prot. 2021;128(2):431–47.

30. El Hazzat N, Adnani M, Msairi S, El Alaoui MA, Mouden N, Chliyeh M, Boughribil S, Selmaoui K, Ouazzani Touhami A, Douira A. Fusarium equiseti as one of the Main Fusarium species causing wilt and root rot of chickpeas in Morocco. Acta Mycol. 2022;57(0).

31. Aymani IE, Qostal S, Mouden N, Selmaoui K, Touhami AO, Benkirane R, Douira A. Fungi associated with saffron (Crocus sativus). Plant Cell Biotechnol Mol Biol.2019; 20(23-24).

32. Van der Lee T, Zhang H, van Diepeningen A, Waalwijk C. Biogeography of Fusarium graminearum species complex and chemotypes: a review. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(4):453–60.

33. Tini F, Covarelli L, Cowger C, Sulyok M, Benincasa P, Beccari G. Infection timing affects Fusarium poae colonization of bread wheat spikes and mycotoxin accumulation in the grain. J Sci Food Agric. 2022;102(14):6358–72.

34. Scherm, B., Balmas, V., Spanu, F., Pani, G., Delogu, G., Pasquali, M., & Migheli, Q. Fusarium culmorum: Causal agent of foot and root rot and head blight on wheat. Mol Plant Pathol. 2012;14(4), 323–341.

35. Infantino A, Belocchi A, Quaranta F, Reverberi M, Beccaccioli M, Lombardi D, Vitale M. Effects of climate change on the distribution of Fusarium spp. in Italy. Sci Total Environ.2023;882:163640.

36. Weather and Climate. Weather and Climate [Online]. [cited 2023 May 25]. Available from: https://weatherandclimate.com

37. Yli-Mattila T, Hussien T, Gavrilova O, Gagkaeva T. Morphological and Molecular Variation Between Fusarium avenaceum, Fusarium arthrosporioides and Fusarium anguioides strains. Pathogens. 2018;7(4):94.

38. Okorski A, Milewska A, Pszczółkowska A, Karpiesiuk K, Kozera W, Dąbrowska JA, Radwińska J. Prevalence of Fusarium fungi and deoxynivalenol levels in winter wheat grain in different climatic regions of Poland. Toxins. 2022;14(2):102.

39. Du M, Ren X, Sun Q, Wang Y, Zhang R. Characterization of Fusarium spp. causing potato dry rot in China and susceptibility evaluation of chinese potato germplasm to the pathogen. Potato Res. 2012;55(2):175–84.

40. Peters JC, Lees AK, Cullen DW, Sullivan L, Stroud GP, Cunnington AC. Characterization of Fusarium spp. responsible for causing dry rot of potato in Great Britain. Plant Pathol. 2008;57(2):262–71.

41. Pee P, Singh M, Prajapati S, Kumari P. Effects of different temperature, pH and relative humidity on the growth of Fusarium oxysporum f. sp. ciceri causing chickpea wilt. Int J Curr Microbiol App Sci. 2020;9(9):1381–8.

42. Gutiérrez-Pozo M, Verheecke-Vaessen C, Kourmpetli S, Terry LA, Medina A. Effect of temperature, relative humidity, and incubation time on the mycotoxin production by Fusarium spp. responsible for dry rot in potato tubers. Toxins. 2024;16(10):414.

43. Chrouqi L, Ouahmane L, Jadrane I, Koussa T, Al Feddy MN. Screening of soil rhizobacteria isolated from wheat plants grown in the Marrakech region (Morocco, North Africa) for plant growth promoting activities. J Mater Environ Sci. 2017;8(9):3382–90.

44. Houmairi H, Oubayoucef A, Idrissi I, Benchekroun Krimi F. High prevalence of Fusarium spp. associated with cereal grains in the central region of Morocco: pathogenic and toxigenic risks. (Haute prévalence de Fusarium spp. associés aux grains de céréales dans la région centrale du Maroc: risques pathogénique et toxinogène). Mar J Agr Vet Sci. 2018;6(3):355–61.

45. Liu Y, Wei X, Chang F, Yu N, Guo C, Cai H. Distribution and Pathogenicity of Fusarium species associated with soybean root rot in Northeast China. Plant Pathol J. 2023;39(6):575–83.

46. Nicol J, Bolat N, Bagci SA, Trethowan RT, William M, Hekimhan H, Yildirim AF, Şahin EZ, Elekcioğlu H, Toktay H, Tunali B, Hede A, Taner S, Braun HJ, Ginkel MV, Keser M, Arisoy ZF, Yorgancilar A, Tülek A, Erdurmus D, Buyuk O, Aydoğdu M. The international breeding strategy for the incorporation of resistance in bread wheat against the soil-borne pathogens (dryland root rot and cyst and lesion cereal nematodes) using conventional and molecular tools. In: Buck HT, Nisi JE, Salomón N, editors. Wheat Production in Stressed Environments. Dordrecht: Springer Netherlands; 2007. p. 125–37.

47. Karim NFA, Mohd M, Nor NMIM, Zakaria L. Saprophytic and potentially pathogenic Fusarium species from peat soil in Perak and Pahang. Trop Life Sci Res. 2016;27(1):1–20.

48. Mohamed Zubi WS, Mohd MH, Mohamed Nor NMI, Zakaria L. Fusarium species in Mangrove Soil in Northern Peninsular Malaysia and the Soil physico-chemical properties. Microorganisms. 2021;9(3):497.

49. Jamil FN, Hashim AM, Yusof MT, Saidi NB. Analysis of soil bacterial communities and physicochemical properties associated with Fusarium wilt disease of banana in Malaysia. Sci Rep. 2022;12(1):999.

50. Ejaz MR, Jaoua S, Ahmadi M, Shabani F. An examination of how climate change could affect the future spread of Fusarium spp. around the world, using correlative models to model the changes. Environ Technol Innov. 2023; 31:103177.

51. Moss MO, Thrane U. Fusarium taxonomy with relation to trichothecene formation. Toxicol Lett. 2004;153(1):23–8.

52. Ekwomadu TI, Mwanza M. Fusarium fungi pathogens, n identification, adverse effects, disease management, and global food security: A review of the latest research. Agriculture. 2023;13(9):1810.

53. Munde S, Dhutraj D, Khaire P, Ghante P. Morphological, cultural and physiological characteristics of pathogen associated with wilt of gladiolus. 2021. In: 4th International Conference on Global Approaches in Natural Resource Management for Climate Smart Agriculture During Pandemic Era of COVID-19; 2021; Ghaziabad, U.P. (India).

54. Bibi A, Mubeen F, Rizwan A, Ullah I, Hammad M, Waqas MAB, Ikram A, Abbas Z, Halterman D, Saeed NA. Morpho-Molecular identification of Fusarium equiseti and Fusarium oxysporum associated with symptomatic wilting of potato from Pakistan. J Fungi. 2024;10(10):701.

55. Vijai Kumar G, Ashok Kumar M, Rajarshi Kumar G. Growth characteristics of Fusarium spp. causing wilt disease in Psidium guajava L. in India. J Plant Prot Res. 2010;50(4):452-62.

56. Pradeep FS, Begam M, Palaniswamy M, Pradeep B. Influence of Culture Media on Growth and Pigment Production by Fusarium moniliforme KUMBF1201 Isolated from Paddy Field Soil. Influence of culture media on growth and pigment production by Fusarium moniliforme KUMBF1201 isolated from paddy field soil. World Appl Sci J. 2013;22(1):70-7.

57. Akter P, Khatun S, Bhowmik DD, Neela FA, Alam N. Vegetative growth and molecular identification of Fusarium equiseti isolated from wilt disease of Centella asiatica L. in Bangladesh. Am J Plant Sci. 2022;13(2):300-10.

58. Saha A, Mandal P, Dasgupta S, Saha D. Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl. J Environ Biol. 2008;29(3):407–410