Unveiling the therapeutic potential of Plumeria pudica: GC-MS Analysis, phytochemical insights, and histopathological evaluation for Wound Healing and Anti-Diabetic applications
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Abstract
The present study investigates the therapeutic potential of Plumeria pudica leaf extract, examining its efficacy as an ointment and herbal formulation for promoting wound healing in albino Wistar rats. The extract's antiseptic and anti-inflammatory properties were evaluated in its application for wound management. This study sought to evaluate the wound-healing efficacy and phytochemical profile of the ethanol extract of Plumeria pudica, employing preliminary screening and Gas Chromatography-Mass Spectrometry (GC-MS) analysis to identify its bioactive compounds. The ethanol extract of Plumeria pudica (EEPP) ointment was formulated using a combination of wool fat, hard paraffin, white soft paraffin, polyethylene glycol, sorbitol monooleate, liquid paraffin, Span-60, Tween-60, methyl hydroxybenzoate, and propyl hydroxybenzoate to create both a herbal mixture and a topical ointment. The wound healing process was monitored over 20 days in excision wound models in rats. The phytochemical composition of the Plumeria pudica extract was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). When treated with a herbal formulation and an ointment containing 5% and 10% Plumeria pudica leaf extract, the excision wound closure rates reached 93.81% and 96.43% by the 14th day of evaluation. On the 20th day post-wounding, the tensile strength of the skin treated with povidone-iodine ointment was 14 ± 3.15 g, whereas rats treated with the topical herbal ointment exhibited tensile strengths of 24 ± 5.11 g and 28 ± 4.05 g. Additionally, GC-MS analysis revealed favourable skin permeability and absorption rates, indicating that the active compounds may contribute to enhanced wound healing. The topical herbal formulation and ointment containing Plumeria pudica leaf extract hold potential for clinical applications in wound healing and diabetes management.
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