Assessment of Antibacterial Activity and Toxicity of Mentha spicata, Mentha piperita, and Mentha pulegium Essential oils
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Abstract
Mentha species are valued for their health benefits, and their essential oils (EOs) are being studied as potential alternatives to synthetic products. This study aimed to evaluate the antibacterial activity and acute oral toxicity of EOs from Mentha spicata, Mentha piperita, and Mentha pulegium. EOs were obtained from the leaves of Mentha spicata and Mentha piperita, and the aerial parts of Mentha pulegium by hydrodistillation. The antibacterial efficacy of the EOs was assessed using disk diffusion and microdilution methods against Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Micrococcus luteus) bacteria. Acute oral toxicity was evaluated following the Organization for Economic Cooperation and Development (OECD) Test No. 423 guideline. The EOs demonstrated varying degrees of antibacterial activity, with M. spicata EO exhibiting the highest efficacy, particularly against S. aureus with inhibition zone diameter of 16.67 mm. No sign of toxicity was observed at a dose of 2000 mg/kg for all three EOs, indicating a favourable safety profile. In conclusion, the bioactive EOs of M. spicata, M. piperita, and M. pulegium show promising antibacterial potential, suggesting their potential use as therapeutic alternatives for bacterial infections. Further evaluations are needed to confirm their safety for human use.
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1. Anwar F, Abbas A, Mehmood T, Gilani A, Rehman N. Mentha: A Genus Rich in Vital Nutra-Pharmaceuticals—A Review. Phytother Res. 2019; 33(10):2548-2570.
2. Mahendran G and Rahman L. Ethnomedicinal, Phytochemical and Pharmacological Updates on Peppermint (Mentha × piperita L.)—A Review. Phytother Res. 2020; 34(9):2088-2139.
3. Mahendran G, Verma SK, Rahman LU. The Traditional Uses, Phytochemistry and Pharmacology of Spearmint (Mentha spicata L.): A Review. J Ethnopharmacol. 2021; 278:114266.
4. Marwa C, Fikri-Benbrahim K, Ou-Yahia D, Farah A. African Peppermint (Mentha piperita) From Morocco: Chemical Composition and Antimicrobial Properties of Essential Oil. J Adv Pharm Technol Res. 2017; 8(3):86-90.
5. Amalich S, Zerkani H, Cherrat A, Soro D, Bourakhouadar M, Mahjoubi M, El Hilali F, Zair T. Study on Mentha pulegium L. From M’rirt (Morocco): Antibacterial and Antifungal Activities of a Pulegone-Rich Essential Oil. J Chem Pharm Res. 2016; 8(5):363-370.
6. El-Gharbaoui A, Benítez G, González-Tejero MR, Molero-Mesa J, Merzouki A. Comparison of Lamiaceae Medicinal Uses in Eastern Morocco and Eastern Andalusia and in Ibn al-Baytar’s Compendium of Simple Medicaments (13th Century CE). J Ethnopharmacol. 2017; 202:208-224.
7. Ait-Sidi-Brahim M, Markouk M, Larhsini M. Moroccan Medicinal Plants as Antiinfective and Antioxidant Agents. In: Ahmad Khan MS, Ahmad I, Chattopadhyay D, editors. New Look to Phytomedicine. Academic Press; 2019. 91-142p.
8. Arrahmouni R, Ouazzani C, Er-Ramly A, Moustaghfir A, Dami A, Ballouch L. Chemical Composition of Moroccan Commercial Essential Oils of Mint: Mentha Spicata, Mentha Piperita, and Mentha Pulegium. Trop J Nat Prod Res. 2023; 7(4):2708-2712.
9. Arrahmouni R, Ouazzani C, Er-Ramly A, Moustaghfir A, Benchama Z, Elamrani A, Benkhouili FZ, El-guourrami O, Dami A, Balouch L. A Comparative Study of the Chemical Composition and Antioxidant Activity of the Essential Oils from Three Species of Mentha Cultivated in Morocco. J Pharm Pharmacogn Res. 2024; 12(6):1021-1039.
10. Bouyahya A, Et-Touys A, Bakri Y, Talbaui A, Fellah H, Abrini J, Dakka N. Chemical Composition of Mentha pulegium and Rosmarinus officinalis Essential Oils and Their Antileishmanial, Antibacterial and Antioxidant Activities. Microb Pathog. 2017; 111:41-49.
11. Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I. Biochemical Activities of Iranian Mentha piperita L. and Myrtus communis L. Essential Oils. Phytochem. 2006; 67(12):1249-1255.
12. Bardaweel SK, Bakchiche B, ALSalamat HA, Rezzoug M, Gherib A, Flamini G. Chemical Composition, Antioxidant, Antimicrobial and Antiproliferative Activities of Essential Oil of Mentha spicata L. (Lamiaceae) From Algerian Saharan Atlas. BMC Complement Altern Med. 2018; 18:201.
13. Luís  and Domingues F. Screening of the Potential Bioactivities of Pennyroyal (Mentha pulegium L.) Essential Oil. Antibiotics. 2021; 10(10):1266.
14. Girard G. The Properties of Essential Oils in Oral Care from Past to Present. Development of a Preclinical Model of Aphthous-Like Oral Lesion to Test the Therapeutic Effects of Essential Oils. Thesis, UHP - Henri Poincaré University. 2010. Available from: https://hal.univ-lorraine.fr/hal-01732627.
15. Lamendin H, Toscano G, Requirand P. Phytotherapy and Aromatherapy in Oral Care. EMC - Dent. 2004; 1(2):179-192.
16. Lanzerstorfer P, Sandner G, Pitsch J, Mascher B, Aumiller T, Weghuber J. Acute, Reproductive, and Developmental Toxicity of Essential Oils Assessed with Alternative in Vitro and in Vivo Systems. Arch Toxicol. 2021; 95(2):673-691.
17. Jaber H, Oubihi A, Ouryemchi I, Boulamtat R, Oubayoucef A, Bourkhiss B, Ouhssine M. Chemical Composition and Antibacterial Activities of Eight Plant Essential Oils from Morocco Against Escherichia coli Strains Isolated from Different Turkey Organs. Biochem Res Int. 2021; 2021:1-9.
18. Ijoma IK and Ajiwe VIE. Jatropha tanjorensis a Flora of Southeast Nigeria: Isolation and Characterization of Naringenin and Validation of Bio-Enhanced Synergistical Activity of α-Tocopherol toward Clinical Isolates of Resistant Bacterial. Makara J Sci. 2022; 26(2):120-127.
19. Ijoma IK and Ajiwe VIE. Antibacterial Activity of Phytochemicals in Ficus thonningii Leaves Extracts against Some Selected Pathogenic Bacterial Prevalent in Sickle Cell Anemia. Jordan J Pharm Sci. 2023; 16(2):345-355.
20. Ijoma IK, Anosike JC, Onwuka C, Njokunwogbu AN, Ajiwe VIE. Phytochemical Constituents of Justicia carnea Leaves and Their Antibacterial Activity. Trop J Nat Prod Res. 2025; 9(1):123-127.
21. Fitsiou E, Mitropoulou G, Spyridopoulou K, Tiptiri-Kourpeti A, Vamvakias M, Bardouki H, Panayiotidis MI. Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata,
Pimpinella anisum and Fortunella margarita. Molecules. 2016; 21(8):1069.
22. Djenane D, Aïder M, Yangüela J, Idir L, Gómez D, Roncalés P. Antioxidant and Antibacterial Effects of Lavandula and Mentha Essential Oils in Minced Beef Inoculated with E. coli O157:H7 and S. aureus During Storage at Abuse Refrigeration Temperature. Meat Sci. 2012; 92(4):667-674.
23. Jebali J, Ghazghazi H, Aouadhi C, ELBini-Dhouib I, Ben Salem R, Srairi-Abid N, Marrakchi N, Rigane G. Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities. Molecules. 2022; 27(1):314.
24. Shahbazi Y. Chemical Composition and in Vitro Antibacterial Activity of Mentha spicata Essential Oil Against Common Food-Borne Pathogenic Bacteria. J Pathog. 2015; 2015:916305.
25. Khoury M, Stien D, Eparvier V, Ouaini N, El Beyrouthy M. Report on the Medicinal Use of Eleven Lamiaceae Species in Lebanon and Rationalization of Their Antimicrobial Potential by Examination of the Chemical Composition and Antimicrobial Activity of Their Essential Oils. Evid Based Complement Altern Med. 2016; 2016:2547169.
26. Mimica-Dukić N, Bozin B, Soković M, Mihajlović B, Matavulj M. Antimicrobial and Antioxidant Activities of Three Mentha Species Essential Oils. Planta Med. 2003; 69(5):413-419.
27. Abdolpour F, Shahverdi AR, Rafii F, Fazeli MR, Amini M. Effects of Piperitone on the Antimicrobial Activity of Nitrofurantoin and on Nitrofurantoin Metabolism by Enterobacter cloacae. Pharm Biol. 2007; 53:406.
28. Zougagh S, Belghiti A, Rochd T, Zerdani I, Mouslim J. Medicinal and Aromatic Plants Used in Traditional Treatment of the Oral Pathology: The Ethnobotanical Survey in the Economic2 Capital Casablanca, Morocco (North Africa). Nat Prod Bioprospect.2019; 9(1):35-48.
29. Moro IJ, Gondo GD, Pierri EG, Rodrigues Pietro RCL, Soares CP, de Sousa DP. Evaluation of Antimicrobial, Cytotoxic and Chemopreventive
Activities of Carvone and Its Derivatives. Braz J Pharm Sci. 2018; 53(4):e00076.
30. Silva BN, Cadavez V, Caleja C, Pereira E, Calhelha RC, Añibarro-Ortega M. Phytochemical Composition and Bioactive Potential of Melissa officinalis L., Salvia officinalis L. and Mentha spicata L. Extracts. Foods. 2023; 12(5):947.
31. Stringaro A, Colone M, Angiolella L. Antioxidant, Antifungal, Antibiofilm, and Cytotoxic Activities of Mentha spp. Essential Oils. Medicines.2018; 5(4):112.
32. Karpiński TM. Essential Oils of Lamiaceae Family Plants as Antifungals. Biomolecules. 2020; 10(1):103.
33. Raghavan R, Devi MPS, Varghese M, Joseph A, Madhavan SS, Sreedevi PV. Effectiveness of Mentha piperita Leaf Extracts against Oral Pathogens: An in Vitro Study. J Contemp Dent Pract. 2018; 19(9):1042-1046.
34. Landeo-Villanueva GE, Salazar-Salvatierra ME, Ruiz-Quiroz JR, Zuta-Arriola N, Jarama-Soto B, Herrera-Calderon O, Pari-Olarte JB, Loyola-Gonzales E. Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an in Vitro Model. Antibiotics. 2023; 12(2):369.
35. Ben Lagha A, Vaillancourt K, Maquera Huacho P, Grenier D. Effects of Labrador Tea, Peppermint, and Winter Savory Essential Oils on Fusobacterium nucleatum. Antibiotics.2020; 9(11):794.
36. Langeveld WT, Veldhuizen EJA, Burt SA. Synergy between Essential Oil Components and Antibiotics: A Review. Crit Rev Microbiol. 2014; 40(1):76-94.
37. Mugisha MK, Ndukui JG, Namutembi A, Waako P, Borg Karlson AK, Vudriko P. Acute and Sub-Acute Toxicity of Ethanolic Leaf Extracts of Rumex abyssinica Jacq. (Polygonaceae) and Mentha spicata L. (Lamiaceae). J Pharm Pharmacol. 2014; 5(3):309-318.
38. Dos Anjos ACP and Isaac A. The Efficacy and Dosage of Mentha piperita Essential Oil in the Control of Monogenean Parasites in Oreochromis niloticus.J Parasit Dis. 2020; 44(3):597-606.
39. Ez-Zriouli R, El Yacoubi H, Imtara H, El-Hessni A, Mesfioui A, Tarayrah M, Mothana RA, Noman OM, Mouhsine F, Rochdi A. Chemical Composition and Antimicrobial Activity of Essential Oils from Mentha pulegium and Rosmarinus officinalis Against Multidrug-Resistant Microbes and Their Acute Toxicity Study. Open Chem. 2022; 20(1):694-702.
40. Jalilzadeh-Amin G and Maham M. Antidiarrheal Activity and Acute Oral Toxicity of Mentha longifolia L. Essential Oil. AvicennaJ Phytomed. 2015; 5(2):128-137.
41. Daneshbakhsh D, Asgarpanah J, Najafizadeh P, Rastegar T, Mousavi Z. Safety Assessment of Mentha mozaffarianii Essential Oil: Acute and Repeated Toxicity Studies. Iran J Med Sci. 2018; 43(5):479-486.