Acquisition of Vermiwash from Eudrilus eugeniae and Its Influence on the Post-Germination Growth of Mustard Greens

Abstract

Vermiwash is a nutrient-rich liquid extract derived from vermicompost, containing essential minerals, enzymes, and plant growth regulators that support plant development. This study aimed to extract vermiwash from Eudrilus eugeniae reared in a substrate enriched with cow manure. The extraction process involved passing water at 15°C (Vermiwash A) and 30°C (Vermiwash B) through layers of sand and gravel to obtain the final vermiwash product. The study further evaluated the effects of these vermiwash on the post-germination growth of mustard green seedlings. On day 10, the mass of Vermiwash A obtained was 318.33 ± 7.64 g, and for Vermiwash B, it was 316.67 ± 11.55 g. By day 25, Vermiwash A exhibited a higher increase in protein content (0.61±0.02 mg/mL) compared to Vermiwash B (0.54±0.02 mg/mL). Vermiwash A also had a higher amino acid content (5.82±0.45 µg/mL) and reducing sugar content (0.58±0.02 mg/mL) than Vermiwash B (4.77±0.86 µg/mL and 0.40±0.03 mg/mL, respectively). Foliar application of vermiwash improved seedling growth compared to both auxin (a plant growth hormone) and the control group. Vermiwash A at a 50% concentration was more effective than Vermiwash B at the same concentration. Seedlings treated with Vermiwash A and NPK fertilizer (on days 7 and 15) showed the best growth, with 7 leaves and a length of 3.18 cm after 21 days. In conclusion, Vermiwash A is more effective than Vermiwash B in enhancing plant growth. These findings suggest that Vermiwash serves as a highly efficient liquid fertilizer, offering a sustainable and eco-friendly alternative for improving crop productivity.