Immunostimulant Effect of Combination Extract of Garcinia mangostana, Moringa oleifera, and Phyllantus niruri based on Phagocytic Index and Titre Antibody Production in Mice
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Abstract
Single-form plants such as Garcinia mangostana, Moringa oleifera folium, and Phyllanthus niruri herb showed immunomodulatory activity. Sefa-Fit or combination extract (CE) is a herbal product that combines three plants (G. mangostana, M. oleifera, and P. niruri), expected to be used as an alternative therapy for immune system disorders. This study aimed to evaluate the in vivo immunostimulant efficacy of Sefa-Fit or combination extract (CE). The research used carbon clearance (for phagocytosis test), antibody titre production using the SRBC (sheep red blood cell) methods, and organ histopathology examination. The results showed that CE showed strong immunostimulant activity based on the phagocytic index parameter at doses of 195 and 390 mg/kg BW to be 1.55±0,08 and 1.51±0,05 (k>1.5). While the dose of 97.5 showed moderate immunostimulant activity (k=1.48). Based on antibody titre testing using the SRBC method, all three doses of CE showed higher antibody titres (1:512; 1:1054, and 1:512), respectively, than the negative control (1:256). The organ histopathology tests showed that damage to the spleen, liver, and kidneys was much less than the negative control (p<0.001). The CE contains secondary metabolites such as the flavonoid, alkaloid, terpenoid, and saponin groups. These findings showed that CE can be developed as an immunostimulant phytotherapy.
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1. Sharma P, Kumar P, Sharma R, Gupta G, Chaudhary A. Immunomodulators: Role of medicinal plants in immune system. Natl J Physiol Pharm Pharmacol. 2017;7(6):552-556. doi:10.5455/njppp.2017.7.0203808032017
2. Kadiyska T, Tourtourikov I, Dabchev K, Zlatarova A, Stoynev N, Hadjiolova R, Spandidos D, Adamaki M, Zoumpourlis V. Herbs and plants in immunomodulation (Review). Int J Funct Nutr. 2023;4(1):1-11. doi:10.3892/ijfn.2023.31
3. Kasemwattanaroj P, Moongkarndi P, Pattanapanyasat K, Mangmool S, Rodpai E, Samer J, Konlata J, Sukapirom K. Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells. Nat Prod Commun. 2013;8(9):1257-1260. doi:10.1177/1934578x1300800919
4. Jayanthi M, Garg SK, Yadav P, Bhatia A, Goel A. Some newer marker phytoconstituents in methanolic extract of Moringa oleifera leaves and evaluation of its immunomodulatory and splenocytes proliferation potential in rats. Indian J Pharmacol. 2015;47(5):518-523. doi:10.4103/0253-7613.165199
5. Adamu UM, Lawal H, Ramasamy R. Immunomodulatory functions of Moringa oleifera (Lam.). Malaysian J Med Heal Sci. 2021;17(7):54-63.
6. Lokapirnasari WP, Lamid M, Kurnijasanti R, Teriyanto N, Kartika AT, Chandra EH, Riong KK, Yulianto AB. Supplementation of synbiotic content of Moringa oleifera extract and Lactobacillus to improve growth performance in starter phase diet of broiler chicken. Trop J Nat Prod Res. 2020;4(12):1096-1100. doi:10.26538/tjnpr/v4i12.11
7. Jantan I, Haque MA, Ilangkovan M, Arshad L. An insight into the modulatory effects and mechanisms of action of Phyllanthus species and their bioactive metabolites on the immune system. Front Pharmacol. 2019;10:1-19. doi:10.3389/fphar.2019.00878
8. Hikmah U, Triastuti A. Mechanism and immunomodulator bioactive compounds of Phyllanthus niruri (meniran). Sci J Pharm. 2022;18(2):205-218. http://journal.uii.ac.id/index.php/JIF
9. Ijoma KI, Ajiwe VII, Ndubuisi JO. Evidence-based preferential in vitro antisickling mechanisme of three native Nigerian plants used in the management of sickle cell disease. Malaysian J Biochem Mol Biol. 2022;3:9-17. http://mjbmb.org
10. Okolie NP, Falodun A, Davids O. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala), and it’s potential for the inhibition of lipid peroxidation. African J Tradit Complement Altern Med. 2014;11(3):221-227. doi:10.4314/ajtcam.v11i3.31
11. Harborne JB. Phytochemical methods a guide to modern techniques of plant analysis. Third Edit. Chapman & Hall, London; 1998.
12. Okafor CE, Ijoma IK, Igboamalu CA, Ezebalu CE, Eze CF, Osita-Chikeze JC, Uzor CE, Ekwuekwe AL. Secondary metabolites, spectra characterization, and antioxidant correlation analysis of the polar and nonpolar extracts of Bryophyllum pinnatum (Lam) Oken. BioTechnologia. 2024;105(2):121-136. doi:10.5114/bta.2024.139752
13. Yadav P, Kumar A, Mahour K, Vihan VS. Phytochemical analysis of some indiegenous plants potent againts endoparasite. J Adv Lab Res Biol. 2010;1(I):56-59.
14. Fadhilah A, Parwati AD, Muflihah CH, Wahyuni AS. Immunomodulatory activity of kasumba turate (Carthamus tinctorius L.) extract against non-specific and specific immune responses in mice. Pharmacon J Farm Indones. 2023;20(1):61-67. doi:10.23917/pharmacon.v20i1.22629
15. Sujono TA, Kamilia D, Kinasih AS. Immune-enhancing effect of ethanol extract of rambutan (Nephelium lappaceum L.) fruit peel on phagocytosis index, paw edema formation, and antibody titer in mice. Trop J Nat Prod Res. 2023;7(9):4038-4042. doi:10.26538/tjnpr/v7i9.28
16. Faradilla M, Iwo MI. Immunomodulatory effect of polysaccharide from white turmeric [Curcuma zedoaria (Christm.) Roscoe)] rhizome. J Ilmu Kefarmasian Indones. 2014;12(2):273-278.
17. Wagner H, Kraus S, Jurcic K. Search for potent immunostimulating agents from plants and other natural sources. In: Wagner H, ed. Immunomodulatory agents from plants. Birkhauser Verlag Basel/Switzerland; 1999:1-39. doi:10.1007/978-3-0348-8763-2_1
18. Palupi DHS, Soemardji AA, Iwo MI. Immunosuppressive effect of methanol extract and ethyl acetate fraction of breadfruit [Artocarpus altilis (Park.) Fosberg] leaves. J Farm Galen (Galenika J Pharmacy). 2022;8(2):124-131. doi:10.22487/j24428744.2022.v8.i2.15946
19. Purkon DB, Iwo MI, Soemardji AA, Rahmawati SF, Fadhlillah FM, Nadhifah A. Immunostimulant activity of Marchantia paleacea Bertol. herb liverwort ethanol extract in BALB/c mice. Indones J Pharm. 2021;32(4):464-473. doi:10.22146/ijp.2128
20. Dillasamola D, Aldi Y, Fakhri M, Diliarosta S, Biomechy Oktomalio P, Noverial. Immunomodulatory effect test from moringa leaf extract (Moringa oleifera L.) with carbon clearance method in male white mice. Asian J Pharm Clin Res. 2018;11(9):241-245. doi:10.22159/ajpcr.2018.v11i9.26703
21. Sujono TA, Fakhrudin DM. Imunomodulator activity of ethanol-70% extract of talok leaves (Muntingia calabura L.) on non specific immune response in male swiss webster mice. Usadha J Pharm. 2024;3(1):104-113. https://jsr.lib.ums.ac.id/index.php/ujp
22. Abbas AK, Lichtman AH, Pillai S. Cellular and molecular immunology e-book. Ninth. Elsevier; 2018.
23. Wahyuningsih SPA, Pramudya M, Putri IP, Savira NII, Winarni D, Suhargo L, Darmanto W. Okra polysaccharides improves spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Biosaintifika J Biol Biol Educ. 2017;9(3):460-465. doi:10.15294/biosaintifika.v9i3.11284
24. Vikasari SN, Wahyuningsih S, Sutjiatmo AB, Razaq A. Specific immunomodulatory effect of water extract of Stachytarpheta jamaicensis herbs. IOP Conf Ser Earth Environ Sci. 2021;755(1):1-5. doi:10.1088/1755-1315/755/1/012010
25. Gholami MH, Rassouli A, Mirzaei S, Hashemi F. The potential immunomodulatory effect of levamisole in humans and farm animals. J Adv Vet Anim Res. 2023;10(4):620-629. doi:10.5455/javar.2023.j717
26. Makiyah SN, Wardhani UH. Citrullus lanatus ethanol extract potential as immunosupressive agent based on spleen histology observation in BALB/c Mice. J Med Brawijaya. 2017;49(4):245-251.
27. Shnayder NA, Ashkhotov A V., Trefilova V V., Nurgaliev ZA, Novitsky MA, Petrova MM, Narodova EA, Al-Zamil M, Chumakova GA, Garganeeva NP, Nasyrova RF. Molecular basic of pharmacotherapy of cytokine imbalance as a component of intervertebral disc degeneration treatment. Int J Mol Sci. 2023;24(9):1-55. doi:10.3390/ijms24097692
28. Sela-Culang I, Kunik V, Ofran Y. The structural basis of antibody-antigen recognition. Front Immunol. 2013;4:1-13. doi:10.3389/fimmu.2013.00302
29. Owen JA, Punt J, Stranford SA. Kuby immunology. Seventh. W.H. Freeman; 2013.
30. Han L, Fu Q, Deng C, Luo L, Xiang T, Zhao H. Immunomodulatory potential of flavonoids for the treatment of autoimmune diseases and tumour. Scand J Immunol. 2022;95(1):1-19. doi:10.1111/sji.13106
31. Vergara-Jimenez M, Almatrafi MM, Fernandez ML. Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidants. 2017;6(4):1-13. doi:10.3390/antiox6040091
32. Chiș A, Noubissi PA, Pop OL, Mureșan CI, Fokam Tagne MA, Kamgang R, Fodor A, Sitar-Tăut AV, Cozma A, Orășan OH, Hegheș SC, Vulturar R, Suharoschi R. Bioactive compounds in Moringa oleifera: mechanisms of action, focus on their anti-inflammatory properties. Plants. 2024;13(20):1-25. doi:10.3390/plants13010020
33. Thorgersen EB, Barratt-Due A, Haugaa H, Harboe M, Pischke SE, Nilsson PH, Mollnes TE. The role of complement in liver injury, regeneration, and transplantation. Hepatology. 2019;70(2):725-736. doi:10.1002/hep.30508
34. Lewis SM, Williams A, Eisenbarth SC. Structure-function of the immune system in the spleen. Hhs Public Access. 2019;123(33):40–43. doi:10.1126/sciimmunol.aau6085.Structure-function
35. Harapko T, Mateshuk-Vatseba L. Submicroscopic changes of structural components of the spleen due to the action of monosodium glutamate. Int Acad J Web Sch. 2020;6 (48):10-16. doi:10.31435/rsglobal